Science advances
2021 Oct 29
Adlanmerini, M;Krusen, BM;Nguyen, HCB;Teng, CW;Woodie, LN;Tackenberg, MC;Geisler, CE;Gaisinsky, J;Peed, LC;Carpenter, BJ;Hayes, MR;Lazar, MA;
PMID: 34705514 | DOI: 10.1126/sciadv.abh2007
[Figure: see text].
Science advances
2021 Dec 17
Wang, CY;Trotter, JH;Liakath-Ali, K;Lee, SJ;Liu, X;Südhof, TC;
PMID: 34919427 | DOI: 10.1126/sciadv.abk1924
[Figure: see text].
Science advances
2021 Dec 17
Lee, JY;Davis, I;Youth, EHH;Kim, J;Churchill, G;Godwin, J;Korstanje, R;Beck, S;
PMID: 34910517 | DOI: 10.1126/sciadv.abj9111
[Figure: see text].
Journal of Virological Methods
2023 Apr 01
Maturana, C;Chan, A;Verpeut, J;Engel, E;
| DOI: 10.1016/j.jviromet.2023.114688
Adeno-associated virus (AAV) has great potential as a source of treatments for conditions that might respond to potent and ubiquitous transgene expression. However, among its drawbacks, the genetic “payload” of AAV vectors is limited to
Science advances
2022 Jan 07
Wang, F;Tan, P;Zhang, P;Ren, Y;Zhou, J;Li, Y;Hou, S;Li, S;Zhang, L;Ma, Y;Wang, C;Tang, W;Wang, X;Huo, Y;Hu, Y;Cui, T;Niu, C;Wang, D;Liu, B;Lan, Y;Yu, J;
PMID: 34995116 | DOI: 10.1126/sciadv.abg5369
[Figure: see text].
Science translational medicine
2021 Dec 01
Sayed, FA;Kodama, L;Fan, L;Carling, GK;Udeochu, JC;Le, D;Li, Q;Zhou, L;Wong, MY;Horowitz, R;Ye, P;Mathys, H;Wang, M;Niu, X;Mazutis, L;Jiang, X;Wang, X;Gao, F;Brendel, M;Telpoukhovskaia, M;Tracy, TE;Frost, G;Zhou, Y;Li, Y;Qiu, Y;Cheng, Z;Yu, G;Hardy, J;Coppola, G;Wang, F;DeTure, MA;Zhang, B;Xie, L;Trajnowski, JQ;Lee, VMY;Gong, S;Sinha, SC;Dickson, DW;Luo, W;Gan, L;
PMID: 34851693 | DOI: 10.1126/scitranslmed.abe3947
[Figure: see text].
Nature neuroscience
2022 Oct 01
Auguste, YSS;Ferro, A;Kahng, JA;Xavier, AM;Dixon, JR;Vrudhula, U;Nichitiu, AS;Rosado, D;Wee, TL;Pedmale, UV;Cheadle, L;
PMID: 36171430 | DOI: 10.1038/s41593-022-01170-x
Oligodendrocyte precursor cells (OPCs) give rise to myelinating oligodendrocytes throughout life, but the functions of OPCs are not limited to oligodendrogenesis. Here we show that OPCs contribute to thalamocortical presynapse elimination in the developing and adult mouse visual cortex. OPC-mediated synapse engulfment increases in response to sensory experience during neural circuit refinement. Our data suggest that OPCs may regulate synaptic connectivity in the brain independently of oligodendrogenesis.
STAR protocols
2022 Jun 17
Nilsson, OR;Kari, L;Rosenke, R;Steele-Mortimer, O;
PMID: 35345596 | DOI: 10.1016/j.xpro.2022.101256
The multilayered meninges surrounding the brain and spinal cord harbor distinct immune cell populations with prominent roles in health and diseases. Here we present an optimized protocol for RNA fluorescence in situ hybridization (RNA FISH) in meningeal whole mounts, allowing the visualization of gene expression. We also describe the combination of this protocol with immunohistochemistry for simultaneous visualization of mRNA and proteins. This protocol can be used for assessing spatial gene expression within the meninges.
Preparation of the intact rodent organ of Corti for RNAscope and immunolabeling, confocal microscopy, and quantitative analysis
STAR Protocols
2021 Jun 01
Reijntjes, D;Breitzler, J;Persic, D;Pyott, S;
| DOI: 10.1016/j.xpro.2021.100544
This protocol describes the preparation of the mouse organ of Corti for RNAscope, immunolabeling, confocal microscopy, and quantitative image analysis to examine transcript and protein localization, sensory hair cells, and synapses. This protocol can be applied to mice and other rodents (juvenile and adult) and can be adapted for other techniques, including electrophysiology and RNA sequencing. This protocol features minimal tissue processing to preserve viability for downstream assays, while isolating the organ of Corti is the most challenging step.
NPJ vaccines
2021 Dec 14
Bhatia, B;Meade-White, K;Haddock, E;Feldmann, F;Marzi, A;Feldmann, H;
PMID: 34907224 | DOI: 10.1038/s41541-021-00416-2
Kyasanur Forest disease virus (KFDV) is a tick-borne flavivirus endemic in India known to cause severe hemorrhagic and encephalitic disease in humans. In recent years, KFDV has spread beyond its original endemic zone raising public health concerns. Currently, there is no treatment available for KFDV but a vaccine with limited efficacy is used in India. Here, we generated two new KFDV vaccine candidates based on the vesicular stomatitis virus (VSV) platform. We chose the VSV-Ebola virus (VSV-EBOV) vector either with the full-length or a truncated EBOV glycoprotein as the vehicle to express the precursor membrane (prM) and envelope (E) proteins of KFDV (VSV-KFDV). For efficacy testing, we established a mouse disease model by comparing KFDV infections in three immunocompetent mouse strains (BALB/c, C57Bl/6, and CD1). Both vaccine vectors provided promising protection against lethal KFDV challenge in the BALB/c model following prime-only prime-boost and immunizations. Only prime-boost immunization with VSV-KFDV expressing full-length EBOV GP resulted in uniform protection. Hyperimmune serum derived from prime-boost immunized mice protected naïve BALB/c mice from lethal KFDV challenge indicating the importance of antibodies for protection. The new VSV-KFDV vectors are promising vaccine candidates to combat an emerging, neglected public health problem in a densely populated part of the world.
