Science advances
2021 Oct 29
Adlanmerini, M;Krusen, BM;Nguyen, HCB;Teng, CW;Woodie, LN;Tackenberg, MC;Geisler, CE;Gaisinsky, J;Peed, LC;Carpenter, BJ;Hayes, MR;Lazar, MA;
PMID: 34705514 | DOI: 10.1126/sciadv.abh2007
[Figure: see text].
Science advances
2021 Dec 17
Wang, CY;Trotter, JH;Liakath-Ali, K;Lee, SJ;Liu, X;Südhof, TC;
PMID: 34919427 | DOI: 10.1126/sciadv.abk1924
[Figure: see text].
Science advances
2021 Dec 17
Lee, JY;Davis, I;Youth, EHH;Kim, J;Churchill, G;Godwin, J;Korstanje, R;Beck, S;
PMID: 34910517 | DOI: 10.1126/sciadv.abj9111
[Figure: see text].
Journal of Virological Methods
2023 Apr 01
Maturana, C;Chan, A;Verpeut, J;Engel, E;
| DOI: 10.1016/j.jviromet.2023.114688
Adeno-associated virus (AAV) has great potential as a source of treatments for conditions that might respond to potent and ubiquitous transgene expression. However, among its drawbacks, the genetic “payload” of AAV vectors is limited to
Science translational medicine
2021 Dec 01
Sayed, FA;Kodama, L;Fan, L;Carling, GK;Udeochu, JC;Le, D;Li, Q;Zhou, L;Wong, MY;Horowitz, R;Ye, P;Mathys, H;Wang, M;Niu, X;Mazutis, L;Jiang, X;Wang, X;Gao, F;Brendel, M;Telpoukhovskaia, M;Tracy, TE;Frost, G;Zhou, Y;Li, Y;Qiu, Y;Cheng, Z;Yu, G;Hardy, J;Coppola, G;Wang, F;DeTure, MA;Zhang, B;Xie, L;Trajnowski, JQ;Lee, VMY;Gong, S;Sinha, SC;Dickson, DW;Luo, W;Gan, L;
PMID: 34851693 | DOI: 10.1126/scitranslmed.abe3947
[Figure: see text].
STAR protocols
2022 Jun 17
Nilsson, OR;Kari, L;Rosenke, R;Steele-Mortimer, O;
PMID: 35345596 | DOI: 10.1016/j.xpro.2022.101256
The multilayered meninges surrounding the brain and spinal cord harbor distinct immune cell populations with prominent roles in health and diseases. Here we present an optimized protocol for RNA fluorescence in situ hybridization (RNA FISH) in meningeal whole mounts, allowing the visualization of gene expression. We also describe the combination of this protocol with immunohistochemistry for simultaneous visualization of mRNA and proteins. This protocol can be used for assessing spatial gene expression within the meninges.
Preparation of the intact rodent organ of Corti for RNAscope and immunolabeling, confocal microscopy, and quantitative analysis
STAR Protocols
2021 Jun 01
Reijntjes, D;Breitzler, J;Persic, D;Pyott, S;
| DOI: 10.1016/j.xpro.2021.100544
This protocol describes the preparation of the mouse organ of Corti for RNAscope, immunolabeling, confocal microscopy, and quantitative image analysis to examine transcript and protein localization, sensory hair cells, and synapses. This protocol can be applied to mice and other rodents (juvenile and adult) and can be adapted for other techniques, including electrophysiology and RNA sequencing. This protocol features minimal tissue processing to preserve viability for downstream assays, while isolating the organ of Corti is the most challenging step.
Nature (2015)
Wang B, Zhao L, Fish M, Logan CY, Nusse R.
PMID: 26245375 | DOI: 10.1038/nature14863
The source of new hepatocytes in the uninjured liver has remained an open question. By lineage tracing using the Wnt-responsive gene Axin2 in mice, we identify a population of proliferating and self-renewing cells adjacent to the central vein in the liver lobule. These pericentral cells express the early liver progenitor marker Tbx3, are diploid, and thereby differ from mature hepatocytes, which are mostly polyploid. The descendants of pericentral cells differentiate into Tbx3-negative, polyploid hepatocytes, and can replace all hepatocytes along the liver lobule during homeostatic renewal. Adjacent central vein endothelial cells provide Wnt signals that maintain the pericentral cells, thereby constituting the niche. Thus, we identify a cell population in the liver that subserves homeostatic hepatocyte renewal, characterize its anatomical niche, and identify molecular signals that regulate its activity.
Science,342(6163), 1226–1230.
Lim X, Tan SH, Koh WL, Chau RM, Yan KS, Kuo CJ, van Amerongen R, Klein AM, Nusse R (2013).
PMID: 24311688 | DOI: 10.1126/science.1239730.
The skin is a classical example of a tissue maintained by stem cells. However, the identity of the stem cells that maintain the interfollicular epidermis and the source of the signals that control their activity remain unclear. Using mouse lineage tracing and quantitative clonal analyses, we showed that the Wnt target gene Axin2 marks interfollicular epidermal stem cells. These Axin2-expressing cells constitute the majority of the basal epidermal layer, compete neutrally, and require Wnt/β-catenin signaling to proliferate. The same cells contribute robustly to wound healing, with no requirement for a quiescent stem cell subpopulation. By means of double-labeling RNA in situ hybridization in mice, we showed that the Axin2-expressing cells themselves produce Wnt signals as well as long-range secreted Wnt inhibitors, suggesting an autocrine mechanism of stem cell self-renewal.
Cell Rep.
2019 Feb 05
Zhang M, Wang Y, Geng J, Zhou S, Xiao B.
PMID: 30726728 | DOI: 10.1016/j.celrep.2019.01.056
Touch and mechanical pain represent distinct, but interactive, modalities of mechanosensation. However, the molecular mechanisms underlying these mechanotransduction processes remain incompletely understood. Here, we show that deletion of the mechanically activated and rapidly adapting Piezo2 channel in a portion of the low-threshold mechanoreceptors and a majority of the IB4-positive nociceptors impairs touch but sensitizes mechanical pain in mice. Ectopic expression of the Piezo2 homolog, the intermediately adapting Piezo1 channel, in sensory neurons can sensitize touch in normal mice and rescue defective touch of the Piezo2-knockout mice. Broad expression of Piezo1 in sensory neurons decreases, rather than evokes, mechanical pain responses. Together, our data suggest that Piezo channels can mediate touch and indirectly suppress acute pain. Tuning Piezo-mediated touch sensitivity allows us to recapitulate the inhibitory effect of touch on acute pain in mouse models.
